Hierarchical clustering of PI3K and MAPK pathway proteins in breast cancer intrinsic subtypes
Copyright policy )Hierarchical clustering of PI3K and MAPK pathway proteins in breast cancer intrinsic subtypes
The phosphatidylinositol‐3‐kinase (PI3K) and mitogen‐activated protein kinase (MAPK) pathways are frequently activated in breast cancer. We recently demonstrated the importance of analyzing multiple proteins as read‐out for pathway activation in ER+/HER2− breast cancer, since single proteins are known to provide insufficient information. Here, we determined pathway activation in other primary breast cancer intrinsic subtypes derived from postmenopausal patients. Tumor blocks were recollected, and immunohistochemistry was performed using antibodies against PTEN, p‐AKT(Thr308), p‐AKT(Ser473), p‐p70S6K, p‐4EBP1, p‐S6RP(Ser235/236) and p‐ERK1/2, followed by unsupervised hierarchical clustering. In 32 ER+/HER2+, 37 ER−/HER2+ and 74 triple‐negative breast cancer patients, subgroups were identified with preferentially activated (A) and preferentially not activated (N) proteins. These subgroups likely reflect tumors with differences in biological behavior as well as treatment outcome.
- Utrecht University Netherlands
- Amsterdam UMC, location VUmc Netherlands
- Amsterdam UMC Netherlands
- Netherlands Heart Institute Netherlands
- Antoni van Leeuwenhoek Hospital Netherlands
mitogen-activated protein kinase, Receptor, ErbB-2, Ribosomal Protein S6 Kinases, 70-kDa, Triple Negative Breast Neoplasms, Original Articles, triple-negative breast cancer, Biomarkers, Tumor, Humans, Female, Mitogen-Activated Protein Kinases, Phosphatidylinositol 3-Kinase, HER2-positive breast cancer, hierarchical clustering, phosphatidylinositol-3-kinase, Proto-Oncogene Proteins c-akt, Breast cancer intrinsic subtypes, Aged, Signal Transduction
mitogen-activated protein kinase, Receptor, ErbB-2, Ribosomal Protein S6 Kinases, 70-kDa, Triple Negative Breast Neoplasms, Original Articles, triple-negative breast cancer, Biomarkers, Tumor, Humans, Female, Mitogen-Activated Protein Kinases, Phosphatidylinositol 3-Kinase, HER2-positive breast cancer, hierarchical clustering, phosphatidylinositol-3-kinase, Proto-Oncogene Proteins c-akt, Breast cancer intrinsic subtypes, Aged, Signal Transduction
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