The aim — to evaluate experimentally the efficacy of the antiseptic gel solution for peritoneal lavage in generalized peritonitis.Materials and methods. 120 Wistar rats weighing 200 — 250 g were the objects of the experimental study. The first stage involved faecal peritonitis modelling via transcutaneous intraperitoneal 2 ml injection of 10 % autologous faecal solution. Depending on the antiseptic solution used for peritoneal lavage, rats were divided into 4 groups: group 1 (n = 20) — 0.02 % chlorhexidine gluconate solution 5 ml, group 2 (n = 20) — 0.02 % decamethoxinum solution 5 ml, group 3 (n = 20) — antiseptic gel solution 5 ml (hyaluronic acid based decamethoxinum), control group 4 (n = 20) — saline solution 5 ml. In 24 hours after peritonitis modelling, laparotomy was performed in all rats under 5 % thiopental sodium anaesthesia and peritoneal effusion culturing was carried out instantly, aimed to determine the dissemination rate, thereafter peritoneal cavity lavage took place. In 48 hours relaparotomy was performed in all rat groups. Parietal and visceral peritonea were assessed visually, peritoneal effusion culturing was carried out intraoperatively, adhesion formation intensity in peritoneal cavity was evaluated.Results and discussion. Combined use of decamethoxinum and hyaluronic acid reveals maximum antiseptic effect comparing to 0.02 % chlorhexidine gluconate (p < 0.01) and have advantage over aqueous based decamethoxinum solution (p > 0.05).Conclusions. The combined use of decamethoxinum antiseptic gel solution and hyaluronic acid have significant advantage as compared with 0.02 % aqueous based chlorhexidine gluconate solution used for peritoneal cavity lavage in generalized faecal peritonitis.