Prolidase-dependent mechanism of (Z)-8,9-epoxyheptadeca-1,11,14-triene-induced inhibition of collagen biosynthesis in cultured human skin fibroblasts
Copyright policy )Prolidase-dependent mechanism of (Z)-8,9-epoxyheptadeca-1,11,14-triene-induced inhibition of collagen biosynthesis in cultured human skin fibroblasts
The effects of polyolefinic compound from roots of Cirsium palustre, (Z)-8,9-epoxyheptadeca-1,11,14-triene (EHT) on collagen biosynthesis, prolidase activity, expression of insulin-like growth factor receptor (IGF-IR), β1 integrin, MAP kinases (pERK1/2), the transcription factors such as nuclear factor kappa B (NF-κB) and hypoxia-inducible factor-1α (HIF-1α) were evaluated in human dermal fibroblasts treated with micromolar concentrations (40-200 μM) for 24 h. It was found that EHT-dependent inhibition of collagen biosynthesis was accompanied by parallel inhibition in prolidase activity. Since IGF-I is the most potent regulator of both processes and prolidase is regulated by β1 integrin signalling, the effect of EHT on IGF-IR and β1 integrin receptor expressions were evaluated. Exposure of the cells to EHT contributed to distinct increase in IGF-IR and slight increase in β1 integrin receptor expressions. It was accompanied by decrease in expression of pERK1/2, HIF-1α and NF-κB. EHT-dependent inhibition of collagen biosynthesis results from inhibition of prolidase activity, the enzyme involved in collagen biosynthesis.
- Wrocław Medical University Poland
- Medical University of Białystok Poland
Dipeptidases, volatile - chemistry, Cells, Epoxy compounds - pharmacology, Polyenes, Alkenes, Fibroblasts - drug effects, Cirsium, Plant Roots, Dipeptidases - metabolism, Receptors, Oils, Volatile, Collagen - biosynthesis, Hypoxia-inducible factor 1, Plant roots - chemistry, Humans, Plant Oils, Insulin-like growth factor I - metabolism, Insulin-Like Growth Factor I, cultured, Extracellular Signal-Regulated MAP Kinases, Alkenes - pharmacology, Cells, Cultured, Molecular Structure, Integrin beta1, NF-kappa B, NF-kappa B - metabolism, Receptors, Somatomedin, Integrin beta1 - metabolism, Polyenes - pharmacology, Fibroblasts, Hypoxia-Inducible Factor 1, alpha Subunit, Extracellular signal-regulated MAP kinases - metabolism, Epoxy Compounds, Fibroblasts - enzymology, Cirsium - chemistry, Collagen, alpha subunit - metabolism, Plant oils - chemistry, Molecular structure, Oils, somatomedin - metabolism
Dipeptidases, volatile - chemistry, Cells, Epoxy compounds - pharmacology, Polyenes, Alkenes, Fibroblasts - drug effects, Cirsium, Plant Roots, Dipeptidases - metabolism, Receptors, Oils, Volatile, Collagen - biosynthesis, Hypoxia-inducible factor 1, Plant roots - chemistry, Humans, Plant Oils, Insulin-like growth factor I - metabolism, Insulin-Like Growth Factor I, cultured, Extracellular Signal-Regulated MAP Kinases, Alkenes - pharmacology, Cells, Cultured, Molecular Structure, Integrin beta1, NF-kappa B, NF-kappa B - metabolism, Receptors, Somatomedin, Integrin beta1 - metabolism, Polyenes - pharmacology, Fibroblasts, Hypoxia-Inducible Factor 1, alpha Subunit, Extracellular signal-regulated MAP kinases - metabolism, Epoxy Compounds, Fibroblasts - enzymology, Cirsium - chemistry, Collagen, alpha subunit - metabolism, Plant oils - chemistry, Molecular structure, Oils, somatomedin - metabolism
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