
Contexte : l'estimation des intervalles post-mortem (PMI) est une étape critique dans les enquêtes sur la mort les plus inédites. After death, bodies begin to decompose in regular patterns which are used to estimate physiological time of death. Ces changements se produisent dans des voies widely variables et aucun facteur unique n'indique de manière accourable le PMI. Objectifs : Ce travail aime comparer entre deux méthodes différentes dans l'estimation de l'intervalle post-mortem quels sont les changements histopathologiques dans la glande suprarénale et le niveau de potassium de l'humour vitré. Materials and methods : The study was conducted on 50 Newzeland male rabbits, at the beginning of the study the rabbits were sacrificed by cervical dislocation then they were classified into 5 groups (I, II, III, IV, V) according to the PMI (0, 6, 12, 18 and 24 hours ) respectivement. Examen histopathologique et histométrique [nombre de cellules chromaffines (cellules /100 μm2), zone de surface médullaire (μm2) et nombre total de cellules chromaffines] pour la gauche suprarénale. Spectroscopic determination of vitreous humor potassium was done. Résultats : Il y avait une augmentation statistique significative dans la valeur moyenne du nombre de cellules de chromaffine (cell/um2) et le niveau de potassium de l'humour vitré (mEq/L) avec PMI augmenté (valeur P was 0.001). On contrast, there was significant statistical decrease in the mean value of the suprarenal medullary area (μm2) and total chromaffin cell count with increased PMI (P-value was 0.001). The results showed significative positive correlation between the vitreous humor potassium level (mEq/L) and chromaffin cell count (cell/100μm2). On the other hand the findings showed significant negative correlation between the vitreous humor potassium level (mEq/L) and the suprarenal medullary area (μm2) and total chromaffin cell (P value was 0.001). Conclusion : le niveau de potassium de l'humour vitré en combinaison avec le nombre de cellules chromaffines (cell/100μm2), la zone médullaire suprarénale et le nombre total de cellules chromaffines peuvent donner une estimation plus précise des intervalles post-mortem. Mots-clés : intervalle post-mortem, gland suprarénal, potassium à l'humour vif.
Antecedentes: La estimación de los intervalos post-mortem (PMI) es un paso crítico en las investigaciones de muerte más desconocidas. After death, bodies begin to decompose in regular patterns which are used to estimate physiological time of death. These changes occur in widely variable ways and no single factor accurately indicates PMI. Objectives: This work aimed to compare between two different methods in estimation postmortem interval which are histopathological changes in suprarenal gland and vitreous humor potassium level. Materials and methods: The study was conducted on 50 Newzeland male rabbits, at the beginning of the study the rabbits were sacrificed by cervical dislocation then they were classified into 5 groups (I, II, III, IV, V) according to the PMI (0, 6, 12, 18 and 24 hours ) respectively. Examen histopatológico e histométrico [recuento de células cromafines (células /100 μm2), área de superficie medular (μm2) y recuento total de células cromafines] para la superposición de la izquierda. Determinación espectroscópica de vitreous humor potassium was done. Results: There was significant statistical increase in the mean value of chromaffin cell count (cell/um2) and the vitreous humor potassium level (mEq/L) with increased PMI (P-value was 0.001). On contrast, there was significant statistical decrease in the mean value of the suprarenal medullary area (μm2) and total chromaffin cell count with increased PMI (P-value was 0.001). The results showed significant positive correlation between the vitreous humor potassium level (mEq/L) and chromaffin cell count (cell/100μm2). On the other hand the findings showed significant negative correlation between the vitreous humor potassium level (mEq/L) and the suprarenal medullary area (μm2) and total chromaffin cell (P value was 0.001). Conclusión: The vitreous humor potassium level in combination with the chromaffin cell count (cell/100μm2), suprarenal medullary area and total chromaffin cell count can give more accurate estimation of the postmortem intervalos. Keywords: postmortem interval, suprarenal gland, vitreous humor potassium.
Background: Estimation of post-mortem intervals (PMI) is a critical step in most un-witnessed death investigations. After death, bodies begin to decompose in regular patterns which are used to estimate physiological time of death. These changes occur in widely variable ways and no single factor accurately indicates PMI. Objectives: This work aimed to compare between two different methods in estimation postmortem interval which are histopathological changes in suprarenal gland and vitreous humor potassium level. Materials and methods: The study was conducted on 50 Newzeland male rabbits, at the beginning of the study the rabbits were sacrificed by cervical dislocation then they were classified into 5 groups (I, II, III, IV, V) according to the PMI (0, 6., 12., 18 and 24 hours ) respectively. Histopathological and histometric examination [chromaffin cell count (cell /100 μm2), medullary surface area (μm2) and total chromaffin cell count] for left suprarenal were done. Spectroscopic determination of vitreous humor potassium was done. Results: There was significant statistical increase in the mean value of chromaffin cell count (cell/um2) and the vitreous humor potassium level (mEq/L) with increased PMI (P-value was 0.001). On contrast, there was significant statistical decrease in the mean value of the suprarenal medullary area (μm2) and total chromaffin cell count with increased PMI (P-value was 0.001). The results showed significant positive correlation between the vitreous humor potassium level (mEq/L) and chromaffin cell count (cell/100μm2). On the other hand the findings showed significant negative correlation between the vitreous humor potassium level (mEq/L) and the suprarenal medullary area (μm2) and total chromaffin cell (P value was 0.001). Conclusion: The vitreous humor potassium level in combination with the chromaffin cell count (cell/100μm2), suprarenal medullary area and total chromaffin cell count can give more accurate estimation of the postmortem intervals. Keywords: postmortem interval, suprarenal gland, vitreous humor potassium.
Background: Estimation of post-mortem intervals (PMI) is a critical step in most un-witnessed death investigations. After death, bodies begin to decompose in regular patterns which are used to estimate physiological time of death. These changes occur in widely variable ways and no single factor accurately indicates PMI. Objectives: This work aimed to compare between two different methods in estimation postmortem interval which are histopathological changes in suprarenal gland and vitreous humor potassium level. Materials and methods: The study was conducted on 50 Newzeland male rabbits, at the beginning of the study the rabbits were sacrificed by cervical dislocation then they were classified into 5 groups (I, II, III, IV, V) according to the PMI (0, 6, 12, 18 and 24 hours ) respectively. Histopathological and histometric examination [chromaffin cell count (cell /100 μm2), medullary surface area (μm2) and total chromaffin cell count] for left suprarenal were done. Spectroscopic determination of vitreous humor potassium was done. Results: There was significant statistical increase in the mean value of chromaffin cell count (cell/um2) and the vitreous humor potassium level (mEq/L) with increased PMI (P-value was 0.001). On contrast, there was significant statistical decrease in the mean value of the suprarenal medullary area (μm2) and total chromaffin cell count with increased PMI (P-value was 0.001). The results showed significant positive correlation between the vitreous humor potassium level (mEq/L) and chromaffin cell count (cell/100μm2). On the other hand, the findings showed significant negative correlation between the vitreous humor potassium level (mEq/L) and the suprarenal medullary area (μm2) and total chromaffin cell (P value was 0.001). Conclusion: The vitreous humor potassium level in combination with the chromaffin cell count (cell/100μm2), suprarenal medullary area and total chromaffin cell count can give more accurate estimation of the postmortem intervals. Keywords: postmortem interval, suprarenal gland, vitreous humor potassium.
خلفية: تقدير فترات ما بعد الوفاة (PMI) هو خطوة حاسمة في معظم التحقيقات في الوفيات التي لم يشهدها أحد. بعد الموت، تبدأ الأجسام في التحلل في أنماط منتظمة تستخدم لتقدير الوقت الفسيولوجي للوفاة. تحدث هذه التغييرات بطرق متغيرة على نطاق واسع ولا يوجد عامل واحد يشير بدقة إلى مؤشر مديري المشتريات. الأهداف: يهدف هذا العمل إلى المقارنة بين طريقتين مختلفتين في تقدير الفاصل الزمني بعد الوفاة وهما التغيرات النسيجية المرضية في الغدة الكظرية ومستوى البوتاسيوم في الفكاهة الزجاجية. المواد والأساليب: أجريت الدراسة على 50 أرانب نيوزيلندية من الذكور، في بداية الدراسة تم التضحية بالأرانب عن طريق خلع عنق الرحم ثم تم تصنيفها إلى 5 مجموعات (I، II، III، IV، V) وفقًا لمؤشر مديري المشتريات (0، 6، 12، 18 و 24 ساعة ) على التوالي. تم إجراء الفحص النسيجي والهيستومتري [عدد خلايا الكرومافين (خلية /100 ميكرومتر مربع)، ومساحة سطح النخاع (ميكرومتر مربع) وإجمالي عدد خلايا الكرومافين] للكظر الأيسر. تم إجراء التحديد الطيفي للخلطة الزجاجية البوتاسيوم. النتائج: كانت هناك زيادة إحصائية كبيرة في متوسط قيمة عدد خلايا الكرومافين (خلية/أم 2) ومستوى البوتاسيوم الفكاهي الزجاجي (ملي مكافئ/لتر) مع زيادة مؤشر مديري المشتريات (كانت قيمة P 0.001). على النقيض من ذلك، كان هناك انخفاض إحصائي كبير في متوسط قيمة المنطقة النخاعية فوق الكلوية (ميكرومتر مربع) وإجمالي عدد خلايا الكرومافين مع زيادة مؤشر مديري المشتريات (كانت قيمة P 0.001). أظهرت النتائج ارتباطًا إيجابيًا كبيرًا بين مستوى البوتاسيوم في الفكاهة الزجاجية (mEq/L) وعدد خلايا الكرومافين (خلية/100 μm 2). من ناحية أخرى، أظهرت النتائج ارتباطًا سلبيًا كبيرًا بين مستوى البوتاسيوم الخلطي الزجاجي (mEq/L) ومنطقة النخاع فوق الكلوي (μm 2) وخلية الكرومافين الكلية (كانت قيمة P 0.001). الاستنتاج: يمكن أن يعطي مستوى البوتاسيوم الخلطي الزجاجي بالاقتران مع عدد خلايا الكرومافين (خلية/100 ميكرومتر مربع) ومنطقة النخاع فوق الكلوي وعدد خلايا الكرومافين الكلي تقديرًا أكثر دقة لفترات ما بعد الوفاة. الكلمات المفتاحية: الفاصل الزمني بعد الوفاة، الغدة الكظرية، البوتاسيوم الفكاهي الزجاجي.
Ecological Impact of Invasive Species on Islands, Spatial Point Patterns in Science, Ecology, Applied Mathematics, Life Sciences, Organic chemistry, Postmortem Interval, Agricultural and Biological Sciences, Chemistry, FOS: Biological sciences, Insect Science, Environmental Science, Physical Sciences, FOS: Mathematics, Potassium, Pathology, Medicine, Emergency medicine, Forensic Entomology and Decomposition Science, Mathematics, Time of death
Ecological Impact of Invasive Species on Islands, Spatial Point Patterns in Science, Ecology, Applied Mathematics, Life Sciences, Organic chemistry, Postmortem Interval, Agricultural and Biological Sciences, Chemistry, FOS: Biological sciences, Insect Science, Environmental Science, Physical Sciences, FOS: Mathematics, Potassium, Pathology, Medicine, Emergency medicine, Forensic Entomology and Decomposition Science, Mathematics, Time of death
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