Aerobic capacity and muscle proteome: Insights from a mouse model
Copyright policy )Aerobic capacity and muscle proteome: Insights from a mouse model
AbstractWe explored the association between aerobic capacity (AC) and the skeletal muscle proteome of McArdle (n = 10) and wild‐type (n = 8) mice, as models of intrinsically ‘low’ and ‘normal’ AC, respectively. AC was determined as total distance achieved in treadmill running until exhaustion. The quadriceps muscle proteome was studied using liquid chromatography with tandem mass spectrometry, with the Search Tool for the Retrieval of Interacting Genes/Proteins database used to generate protein–protein interaction (PPI) networks and enrichment analyses. AC was significantly associated (P‐values ranging from 0.0002 to 0.049) with 73 (McArdle) and 61 (wild‐type) proteins (r‐values from −0.90 to 0.94). These proteins were connected in PPI networks that enriched biological processes involved in skeletal muscle structure/function in both groups (false discovery rate <0.05). In McArdle mice, the proteins associated with AC were involved in skeletal muscle fibre differentiation/development, lipid oxidation, mitochondrial function and calcium homeostasis, whereas in wild‐type animals AC‐associated proteins were related to cytoskeleton structure (intermediate filaments), cell cycle regulation and endocytic trafficking. Two proteins (WEE2, THYG) were associated with AC (negatively and positively, respectively) in both groups. Only 14 of the 132 proteins (∼11%) associated with AC in McArdle or wild‐type mice were also associated with those previously reported to be modified by aerobic training in these mice, providing preliminary evidence for a large divergence in the muscle proteome signature linked to aerobic training or AC, irrespective of AC (intrinsically low or normal) levels. Our findings might help to gain insight into the molecular mechanisms underlying AC at the muscle tissue level.
Male, ANATOMY::Musculoskeletal System::Muscles::Muscle, Skeletal, 577.2, Proteome, Physiology, proteome, Bioquímica (Farmacia), PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Physical Endurance::Exercise Tolerance, ANATOMÍA::sistema musculoesquelético::músculos::músculo esquelético, Muscle Proteins, COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteoma, Mice, FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::resistencia física::tolerancia al ejercicio, 32 Ciencias Médicas, Physical Conditioning, Animal, QP1-981, Animals, Protein Interaction Maps, Muscle, Skeletal, 577.1, Rates (Animals de laboratori), Proteoma, Sistema cardiovascular, COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas contráctiles::proteínas musculares, endurance, glycogenosis type V, cardiorespiratory fitness, exercise, Proteínas Musculares, Medicina deportiva, Aparato respiratorio, Proteïnes muscular, omics, Mice, Inbred C57BL, PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Movement::Motor Activity::Exercise::Physical Conditioning, Animal, Múscul estriat, CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Proteome, Biología molecular (Farmacia), ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice, CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Contractile Proteins::Muscle Proteins, ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratas, Condició física, FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::movimiento::actividad motora::ejercicio físico::acondicionamiento físico animal, Sistema Musculoesquelético, Research Article
Male, ANATOMY::Musculoskeletal System::Muscles::Muscle, Skeletal, 577.2, Proteome, Physiology, proteome, Bioquímica (Farmacia), PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Physical Endurance::Exercise Tolerance, ANATOMÍA::sistema musculoesquelético::músculos::músculo esquelético, Muscle Proteins, COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteoma, Mice, FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::resistencia física::tolerancia al ejercicio, 32 Ciencias Médicas, Physical Conditioning, Animal, QP1-981, Animals, Protein Interaction Maps, Muscle, Skeletal, 577.1, Rates (Animals de laboratori), Proteoma, Sistema cardiovascular, COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas contráctiles::proteínas musculares, endurance, glycogenosis type V, cardiorespiratory fitness, exercise, Proteínas Musculares, Medicina deportiva, Aparato respiratorio, Proteïnes muscular, omics, Mice, Inbred C57BL, PHENOMENA AND PROCESSES::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Movement::Motor Activity::Exercise::Physical Conditioning, Animal, Múscul estriat, CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Proteome, Biología molecular (Farmacia), ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice, CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Contractile Proteins::Muscle Proteins, ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratas, Condició física, FENÓMENOS Y PROCESOS::fenómenos fisiológicos nerviosos y musculoesqueléticos::fenómenos fisiológicos musculoesqueléticos::movimiento::actividad motora::ejercicio físico::acondicionamiento físico animal, Sistema Musculoesquelético, Research Article
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