descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 16 Nov 2013 English Publisher:American Diabetes AssociationJournal:Diabetes, volume 62, pages 4,266-4,269 (issn: 0012-1797, eissn: 1939-327X,

Authors: Acosta, Lourdes; Hmadcha, Abdelkrim; Escacena, Natalia; Pérez-Camacho, Inmaculada; de la Cuesta, Antonio; Ruiz-Salmeron, Rafael; Gauthier, Benoit R; +1 Authors

doi: 10.2337/db13-0896

pmid: 24043757

pmc: PMC3837061

handle: 10668/2263 , 10261/122578

Adipose Mesenchymal Stromal Cells Isolated From Type 2 Diabetic Patients Display Reduced Fibrinolytic Activity

- Summary
- Subjects
- Related research
  (4)
- Metrics

Abstract

Stem cells have been successfully used for the treatment of critical limb ischemia (CLI). We conducted a clinical trial to determine the feasibility of using autologous adipose-derived mesenchymal stromal cells (AdMSCs) for the treatment of CLI. Unexpectedly, two diabetic patients developed peripheral microthrombosis. This adverse effect, which contrasts with the reported antithrombotic properties of MSCs, may stem from the diabetic environment that alters the fibrinolytic activity of AdMSCs, thereby increasing the probability of developing thrombosis. Here, we confirm this premise by demonstrating that diabetic AdMSCs cultured in the presence of blood sera expressed and released higher levels of plasminogen activator inhibitor type 1, reduced levels of tissue plasminogen activator, and lower d-dimer formation compared with nondiabetic AdMSCs. Thus, to establish an appropriate cell therapy for diabetic patients, we recommend including new preclinical safety tests, such as the d-dimer and/or the tissue plasminogen activator-to-plasminogen activator inhibitor type 1 ratio tests, to assess fibrinolytic activity of cells before implantation.

Related Organizations

Keywords

Medical Subject Headings::Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus, Type 2, Adult, Male, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over, Adolescent, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Medical Subject Headings::Chemicals and Drugs::Biological Factors::Blood Coagulation Factors::Plasminogen Inactivators::Plasminogen Activator Inhibitor 1, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, Plasminogen Activator Inhibitor 1, Humans, Medical Subject Headings::Chemicals and Drugs::Biological Factors::Blood Coagulation Factors::Tissue Plasminogen Activator, Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue, Medical Subject Headings::Named Groups::Persons::Age Groups::Adolescent, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, Original Research, Aged, Aged, 80 and over, Fibrinolysis, Medical Subject Headings::Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Blood Physiological Phenomena::Blood Physiological Processes::Hemostasis::Blood Coagulation::Fibrinolysis, Medical Subject Headings::Check Tags::Female, Mesenchymal Stem Cells, Middle Aged, Medical Subject Headings::Check Tags::Male, Adipose Tissue, Diabetes Mellitus, Type 2, Tissue Plasminogen Activator, Female, Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells

4 Research products, page 1 of 1

Nutrigenetics and Nutrigenomics Insights into Diabetes Etiopathogenesis
2014IsAmongTopNSimilarDocuments
Development of a cell-based medicinal product: regulatory structures in the European Union
2012IsAmongTopNSimilarDocuments
Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment
2013IsAmongTopNSimilarDocuments
Standard Requirement of a Microbiological Quality Control Program for the Manufacture of Human Mesenchymal Stem Cells for Clinical Use
2014IsAmongTopNSimilarDocuments

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	60
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%