A redox-dependent dimerization switch regulates activity and tolerance for reactive oxygen species of barley seed glutathione peroxidase
Copyright policy )A redox-dependent dimerization switch regulates activity and tolerance for reactive oxygen species of barley seed glutathione peroxidase
Monomeric and dimeric forms of recombinant barley (Hordeum vulgare subsp. vulgare) glutathione peroxidase 2 (HvGpx2) are demonstrated to display distinctly different functional properties in vitro. Monomeric HvGpx2 thus has five fold higher catalytic efficiency than the dimer towards tert-butyl hydroperoxide, but is more sensitive to inactivation by hydrogen peroxide. Treatment of the monomer with hydrogen peroxide results in dimer formation. This observed new behavior of a plant glutathione peroxidase suggests a mechanism involving a switch from a highly catalytically competent monomer to a less active, but more oxidation-resistant dimer.
- University of Southern Denmark Denmark
- Technical University of Denmark Denmark
- Copenhagen University Hospital Denmark
Physiological, tert-Butylhydroperoxide, Reactive Oxygen Species/metabolism, Oligomerization, Alkyl peroxide, Adaptation, Hordeum/enzymology, Thioredoxin, Hydrogen Peroxide/metabolism, Seeds/metabolism, Glutathione Peroxidase, Glutathione Peroxidase/metabolism, Peroxidases/metabolism, Hordeum, Hydrogen Peroxide, Peroxiredoxins, Hydrogen peroxide, Adaptation, Physiological, Glutathione, Glutathione/metabolism, Oxidative Stress, Peroxidases, Seeds, Glutathione peroxidase, Peroxiredoxins/metabolism, Antioxidant, Reactive Oxygen Species, tert-Butylhydroperoxide/metabolism, Dimerization, Oxidation-Reduction
Physiological, tert-Butylhydroperoxide, Reactive Oxygen Species/metabolism, Oligomerization, Alkyl peroxide, Adaptation, Hordeum/enzymology, Thioredoxin, Hydrogen Peroxide/metabolism, Seeds/metabolism, Glutathione Peroxidase, Glutathione Peroxidase/metabolism, Peroxidases/metabolism, Hordeum, Hydrogen Peroxide, Peroxiredoxins, Hydrogen peroxide, Adaptation, Physiological, Glutathione, Glutathione/metabolism, Oxidative Stress, Peroxidases, Seeds, Glutathione peroxidase, Peroxiredoxins/metabolism, Antioxidant, Reactive Oxygen Species, tert-Butylhydroperoxide/metabolism, Dimerization, Oxidation-Reduction
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