Mine microseismic signal denoising is a basic and crucial link in microseismic data processing, which influences the accuracy and reliability of the monitoring system, and is of great significance with regard to safety during mining. Therefore, this study introduces a deep learning method to improve the mapping function and sparsity of signals in the time-frequency domain and constructs a denoising framework based on a deep convolutional autoencoder to address the denoising problem of mine microseismic signals. First, all noisy microseismic signals are normalized to ensure the nonlinear expression ability of the constructed denoising framework. Then, the normalized signals are transformed into the time-frequency domain using the short-time Fourier transform (STFT), and the real and imaginary parts of time-frequency coefficients serve as the input of the deep convolutional autoencoder to output the masks of the effective and noise signals. Next, these masks are applied to the time-frequency coefficients of the noisy microseismic signals, and the time-frequency coefficients of the potentially effective and noise signals are estimated. Finally, inverse STFT is used to transform these time-frequency coefficients to the time domain to obtain the final denoised effective and noise signals. The constructed framework automatically learns rich features from synthetic data to separate the effective and noise signals, thereby achieving the purpose of fast and automatic denoising. The experimental results show that compared with the wavelet threshold and ensemble empirical mode decomposition, the denoising framework considerably improves the signal-to-noise ratio of mine microseismic signals with less waveform distortion. Moreover, it can achieve a better denoising effect efficiently even in the case of a low SNR, which has obvious advantages. The constructed denoising framework is suitable for microseismic monitoring signals of various mine dynamic disasters and provides strong technical support for intelligent monitoring and early warning concerning production risks in mines.