A comparative study of the preventative effects exerted by two probiotics,Lactobacillus reuteriandLactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis
Copyright policy )A comparative study of the preventative effects exerted by two probiotics,Lactobacillus reuteriandLactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis
The intestinal anti-inflammatory effects of two probiotics isolated from breast milk,Lactobacillus reuteriandL. fermentum, were evaluated and compared in the trinitrobenzenesulfonic acid (TNBS) model of rat colitis. Colitis was induced in rats by intracolonic administration of 10 mg TNBS dissolved in 50 % ethanol (0·25 ml). EitherL. reuteriorL. fermentumwas daily administered orally (5 × 108colony-forming units suspended in 0·5 ml skimmed milk) to each group of rats (n10) for 3 weeks, starting 2 weeks before colitis induction. Colonic damage was evaluated histologically and biochemically, and the colonic luminal contents were used for bacterial studies and for SCFA production. Both probiotics showed intestinal anti-inflammatory effects in this model of experimental colitis, as evidenced histologically and by a significant reduction of colonic myeloperoxidase activity (P < 0·05).L. fermentumsignificantly counteracted the colonic glutathione depletion induced by the inflammatory process. In addition, both probiotics lowered colonic TNFα levels (P < 0·01) and inducible NO synthase expression when compared with non-treated rats; however, the decrease in colonic cyclo-oxygenase-2 expression was only achieved withL. fermentumadministration. Finally, the two probiotics induced the growth of Lactobacilli species in comparison with control colitic rats, but the production of SCFA in colonic contents was only increased whenL. fermentumwas given. In conclusion,L. fermentumcan exert beneficial immunomodulatory properties in inflammatory bowel disease, being more effective thanL. reuteri, a probiotic with reputed efficacy in promoting beneficial effects on human health.
Diarrhea, Limosilactobacillus reuteri, Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestinal Mucosa, Limosilactobacillus fermentum, Colon, Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Gland, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal, Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestine, Large::Colon, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Digestive::Diarrhea, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus reuteri, Feces, Medical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Volatile, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ Size, Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase, Medical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha, Animals, Intestinal Mucosa, Muscle, Skeletal, Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Supplements::Probiotics, Peroxidase, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Oligopeptides::Glutathione, Medical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Intestinal Diseases::Inflammatory Bowel Diseases, Medical Subject Headings::Anatomy::Fluids and Secretions::Feces, Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Hydrogen-Ion Concentration, Medical Subject Headings::Anatomy::Musculoskeletal System::Muscles::Muscle, Skeletal, Probiotics, Medical Subject Headings::Check Tags::Female, Organ Size, Hydrogen-Ion Concentration, Colitis, Fatty Acids, Volatile, Inflammatory Bowel Diseases, Glutathione, Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Nitro Compounds::Nitrobenzenes::Trinitrobenzenes::Trinitrobenzenesulfonic Acid, Gastrointestinal Contents, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus fermentum, Rats, Medical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Gastroenteritis::Colitis, Medical Subject Headings::Organisms::Eukaryota::Animals, Models, Animal, Female, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats, Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Spleen, Medical Subject Headings::Anatomy::Fluids and Secretions::Gastrointestinal Contents
Diarrhea, Limosilactobacillus reuteri, Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestinal Mucosa, Limosilactobacillus fermentum, Colon, Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Gland, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal, Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestine, Large::Colon, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Digestive::Diarrhea, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus reuteri, Feces, Medical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Volatile, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ Size, Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase, Medical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha, Animals, Intestinal Mucosa, Muscle, Skeletal, Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Supplements::Probiotics, Peroxidase, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Oligopeptides::Glutathione, Medical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Intestinal Diseases::Inflammatory Bowel Diseases, Medical Subject Headings::Anatomy::Fluids and Secretions::Feces, Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Hydrogen-Ion Concentration, Medical Subject Headings::Anatomy::Musculoskeletal System::Muscles::Muscle, Skeletal, Probiotics, Medical Subject Headings::Check Tags::Female, Organ Size, Hydrogen-Ion Concentration, Colitis, Fatty Acids, Volatile, Inflammatory Bowel Diseases, Glutathione, Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Nitro Compounds::Nitrobenzenes::Trinitrobenzenes::Trinitrobenzenesulfonic Acid, Gastrointestinal Contents, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus fermentum, Rats, Medical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Gastroenteritis::Colitis, Medical Subject Headings::Organisms::Eukaryota::Animals, Models, Animal, Female, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats, Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Spleen, Medical Subject Headings::Anatomy::Fluids and Secretions::Gastrointestinal Contents
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