Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis
Copyright policy )Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis
ABSTRACT The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.
- Mater Health Services Australia
- University Hospital Southampton NHS Foundation Trust United Kingdom
- Mater Adult Hospital Australia
- Kings College London, University of London United Kingdom
- University of Queensland Australia
Adult, DNA, Bacterial, Diversity, Mouth, Bacteria, Cystic Fibrosis, Sputum, 610, Genes, rRNA, Bacterial Infections, DNA, Ribosomal, RNA, Ribosomal, 16S, Lung-Infections, Cluster Analysis, Humans, Lung, Polymorphism, Restriction Fragment Length
Adult, DNA, Bacterial, Diversity, Mouth, Bacteria, Cystic Fibrosis, Sputum, 610, Genes, rRNA, Bacterial Infections, DNA, Ribosomal, RNA, Ribosomal, 16S, Lung-Infections, Cluster Analysis, Humans, Lung, Polymorphism, Restriction Fragment Length
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