INTRODUCTION: Despite numerous studies and the existence of many recommendations regarding infusion therapy in newborns, the need for subsidies and precise dosing of intraoperative glucose for children of the neonatal period has not been determined. OBJECTIVES: Analysis of the intraoperative glycemic profile in newborns and determination of the need for parenteral glucose subsidies during surgery in this category of patients. MATERIAL AND METHODS: The study included 101 newborn children under 28 days of age with surgical diseases requiring surgical treatment in the neonatal period. The study was approved by the local ethics committee of the Russian National Research Medical University named after. N.I. Pirogov reference number 192 dated January 27, 2020. Demographic and clinical data were assessed using nonparametric statistics and presented as median values with interquartile ranges. Continuous variables were compared using one-way analysis of variance. RESULTS: In the absence of a planned subsidy of glucose intraoperatively, 74.5 % of children have hypoglycemia < 2.9 mmol/l, 55.3 % have blood sugar < 2.6 mmol/l, especially at the stages of intubation and skin incision. With planned titration of glucose at a dose of 2.3 mg/kg/min, hypoglycemia is recorded almost 2 times less often (40 % of children) (p = < 0.001), a third of children had hyperglycemia (16.7 %) and a hyperosmolar state (50 %). When glucose was administered at a dose of 1.15 mg/kg/min, more children had euglycemia of 55 %, p = 0.038. CONCLUSIONS: A statistically significant difference in glycemia during surgery in children was revealed depending on the intraoperative glucose supplement. The need of glucose is individual and depends on many factors.