Territory of Primorsk is endemic for tularemia, leptospirosis, hemorrhagic fever with renal syndrome, and tick-borne infections. Human (146) and animal (373) samples were examined by bacteriological, serological and molecular-genetic methods. Seroprevalence in humans is weakly expressed except tularemia (14.0-40.4%). Lack of tularemia antibody in cattle (n = 40) indicates low significance of blood-sucking insects as the infection vectors. Small mammals (n = 333) were infected with Francisella (34.1%), Leptospira (38.4%) and Hantaviruses of Puumala and Hantaan genotypes (8.7%). Two cultures: L. borgpetersenii and L. kirschneri serogroups Javanica and Grippotyphosa were isolated. High activity of the infection natural foci requires appropriate preventive measures.