Azo dye (1), that characterized (Z)-5-amino-2-(((4-(5-(p-tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl) phenyl) sulfonyl) diazenyl) phenol) was prepared. Then characterized using m.p., IR, UV-visible, mass spectrum and elemental analysis (CHN). Analytical studies approved on this dye; which showed high solubility in ethanol and gave three isopiestic points, that when studying the pH effect in a range of buffer solutions. The protonation constants pKb1 and pKb2 of nitrogen atom and the ionization constant pKa of OH-group were equal to 3.4, 7.26 and 10.5 respectively. These results indicated the suggested ionization scheme in acidic and basic media. However, due to recommend the new azo dye as novel medicine or chemical sanitizer especially in dual infection. the complex of azo dye (1) with Zn was decreased the biological activity of dye.