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Acta Scientiarum: Agronomy
Article . 2012
Data sources: DOAJ
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Multiplication of embryogenic calli in Coffea arabica L.=Indução e multiplicação de calos embriogênicos de Coffea arabica L.

Authors: João Batista Teixeira; Ana Carolina Ramia Santos; Moacir Pasqual; Carlos Henrique Siqueira de Carvalho; Juliana Costa de Rezende;

Multiplication of embryogenic calli in Coffea arabica L.=Indução e multiplicação de calos embriogênicos de Coffea arabica L.

Abstract

The goal of this project was to evaluate the embryogenic callus induction of two Coffea arabica clones selected for their characteristics of rust resistance and high yield, as well as to compare their multiplication in two different media under both solid and liquid cultivation conditions. The protocol described by Teixeira et al. (2004) was used for callus induction in a randomized block design in which each clone was considered a treatment. Evaluation of callus induction was carried out 180 days after initiation by counting embryogenic calli. For callus multiplication, the treatments consisted of two different media [stage two of Albarran et al. (2004) and the multiplication medium described by Teixeira et al. (2004)] and two cultivation systems (solid and liquid). Evaluations were conducted by weighing calli 21, 42 and 63 days after initiation of the experiment. The two studied clones exhibited the same potential for embryogenic callus induction. The potential for embryogenic callus multiplication was influenced by the plant’s genotype. When compared with the liquid system, the solid system displayed the highest level of embryogenic callus multiplication for the clones studied.Este estudo foi realizado com o objetivo de avaliar a indução de calos embriogênicos de dois clones de Coffea arabica, selecionados para resistência à ferrugem e de alta produtividade e comparar a multiplicação desses calos em dois meios de cultura nos sistemas de cultivo gelatinoso e líquido. Para a indução de calos foi utilizado o protocolo descrito por Teixeira et al. (2004), em delineamento experimental inteiramente casualizado, sendo cada clone considerado um tratamento. A avaliação deste experimento foi realizada 180 dias após a instalação, por meio da contagem de calos embriogênicos formados. Para a multiplicação de calos, os tratamentos constituíram-se de dois meios de cultura (meio do estágio dois de Albarran et al. (2004) e meio de multiplicação de Teixeira et al. (2004)) e dois sistemas de cultivo (gelatinoso e líquido). As avaliações foram realizadas aos 21, 42 e 63 dias após a instalação do experimento, por meio da pesagem dos calos. Verificou-se que os clones avaliados apresentam o mesmo potencial de formação de calos embriogênicos. O potencial de multiplicação de calos embriogênicos é influenciado pelo genótipo. O sistema gelatinoso apresentou maior eficiência na multiplicação de calos embriogênicos dos clones estudados quando comparado ao sistema líquido.

Keywords

culture media, sistema de cultivo, meio de cultura, genótipo., genotype, Agriculture (General), cultivation systems, S1-972

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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