Deoxynivalenol （DON）， also known as vomitoxin （VT）， is one of the most common mycotoxins produced by Fusarium spp. It is widely distributed in cereal and related products， which can cause huge economic losses to the world food production and poses a major threat to human and animal health. At present， the method of biological detoxification using microorganisms and enzymes shows good application prospects. Many microorgranisms， including bacteria and fungi， have the ability to absorb or degrade mycotoxins. This article outlines the occurrence and toxicity of DON in food， as well as the mechanism of biological detoxification of DON. Then， the recent progress in the detoxification of DON using fungi， bacteria and plants is summarized in more detail. Biological agents relating to DON detoxification are also discussed， with the aim of providing a reference for biocontrol of DON in food and feed.