Los resultados muestran un valor de heterocigosidad de 11% si se considera los 12 loci presuntivos analizados. El polimorfismo es de 25%, semejante al reportado en distintas especies de peces marinos. Por otro lado el análisis estadístico de las pruebas Fst y X² sugieren que las poblaciones de Valparaíso y Lebu se ajustan al equilibrio de Hardy-Weinberg. Los valores de Identidad genética (I = 0,996) y Distancia genética de Nei (D = 0,004), demuestran diferencias no significativasThe pacific pomfret, Brama australis, is an epipelagic and mesopelagic fish and the distribution is from Coquimbo in the north to the Austral zone in the south. The main fisheries area are the V and VIII Región. We study the genetic variability in this species using starch gel electrophoresis of proteins. The samples com from Valparaíso (33°05'S; 71°40'W) and from Lebu (37°37'S; 73°40''`W). The results show that the heterozygosity is 11% when 12 presuntive loci are analized. The polymorphism is 25 %, similar to those reported in different species of marine fish. On the other hand, the statistical analysis of Fst and X² test suggest that populations of Valparaíso and Lebu fit to Hardy-Weinberg equilibrium. The values of genetic identity (I = 0.996) and Nei genetic distance (D = 0,004) demonstrate not significative differences