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Boletim do Instituto de Pesca
Article . 2018
Data sources: DOAJ
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PRESERVAÇÃO DE Chlorella sp POR RESFRIAMENTO E CONGELAMENTO

Authors: Márcia Santos Nunes GALVÃO; Celina Maria Marcondes PIMENTAL; Ronaldo Almeida da SILVA; Naoyo YAMANAKA;

PRESERVAÇÃO DE Chlorella sp POR RESFRIAMENTO E CONGELAMENTO

Abstract

Culturas de Chlorella sp, previamente centrifugadas e não centrifugadas, foram mantidas em refrigerador e em "freezer" (sem crioprotetor), a fim de verificar a viabilidade de sua preservação por perí­­odos de 1 a 28 meses. Constatou-se que a espécie resiste í­Â estocagem tanto por congelamento como por resfriamento por perí­­odos de até 28 meses. No entanto, culturas de material resfriado e previamente centrifugado apresentaram maior crescimento populacional que as submetidas aos demais tratamentos, tendo atingido a densidade de 10 x 106 células/mL em poucos dias após cada perí­­odo de estocagem. A fase "lag" foi mais longa quanto maior o perí­­odo de estocagem, para todos os tratamentos testados, sendo que após o 9º mês de estocagem a reativação tornou-se sensivelmente mais lenta. A preservação de culturas centrifugadas em refrigerador comum é um método simples e de utilidade na aquicultura, pois permite a estocagem de grande número de células em pequeno volume.

Keywords

resfriamento, Chlorella sp, congelamento, Aquaculture. Fisheries. Angling, SH1-691, centrifugação, preservação

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold