In recent years, there is a great tendency for ecological dyeing and finishing of natural fabrics with natural dyes. Natural dyes obtained from nature were used for dyeing of textiles from ancient times till the nineteenth century. Weld (Reseda Luteola L.) produces the most stable yellow colour and shades and Madder (Rubia tinctorum L.) produces the most beautiful red colour and shades. These natural dyes were usually combined toghether to produce different shades of orange. Colour represents the bridge between science-art- industry, and can be described by various methods, with the following features: hue, saturation or purity (chroma) and brightness. Any colour can be defined by these three values which describe a unique color. The paper presents the colour analysis, resulting 16 variants of the ecological dyeing on hemp fabric with natural dyes Weld and Madder. In the colour analysis, the following was observed: the influence of the prior application of treatments for improving the absorption capacity of the dye (pre-treatments with enzyme and scouring and bleaching in one step), the influence of using different combinations and concentrations of dye (Weld-Madder combination and Madder-Weld combination), but also the colour resistance after washing on the tested samples. In order to have a general view over the colours obtained after dyeing with natural dyes on hemp samples, colouring measurements in the CIE LAB system with spectrophotometer 968 (X– Rite, USA), with light source D65 and observer of 100 (Enhanced polarization filter according to ISO/ DIS 13655) have been carried out.