Objective: To determine the Chlamydia trachomatis IgG antibodies in females of reproductive age visiting Holy Family Hospital, Rawalpindi Study Design: Comparative cross-sectional Study. Place and Duration of Study: Microbiology department, Holy Family Hospital (HFH), Rawalpindi Medical College, Rawalpindi from Apr 2014 to Oct 2014. Methodology: A total of 328 females of reproductive age with 164 each in both fertile and infertile group, visiting Holy Family Hospital were included in the study. After taking written consent, detailed history was taken and recorded on bio data proforma. Blood sample was taken in Gynaecology outpatient department and then transported to Microbiology Department, Holy Family Hospital. IgG antibodies were detected by Enzyme-linked immunosorbent assay (ELISA). The data was recorded & analyzed by SPSS version 20. Results: Out of 328 females, 37 (11.28%) were positive for Chlamydia trachomatis IgG antibodies. Out of this, 8.5% were fertile females and 14% were infertile. Conclusion: This study showed the high frequency of Chlamydia trachomatis IgG antibodies in females of Rawalpindi.