During scientific-industrial experiment influence of biopreparation STF 1/56 Tim made on the basis of life culture Enterococcus faecium 1/56 on newborn calves of red steppe bred was studied since 2-day age. Animals of test group take preparation daily with milk at doze of 5 ml/head (up to 15-day age) and 10 mg/head (15.20-day age). Animals of control group do not take a preparation. Total amount of eritrocites, leukocites in Goryaev’s count camera. Leucogram was get in smears stained after Romanovsky-Gimza. To determine the bactericidal activity of blood was performed staging of spontaneous and stimulated recovery test nitro blue tetrazolium (NBT-test). At 15-th day of study there were statistically significant (р < 0.001) differences in number of eosinophils (7.09%) and stab neutrophils (13.3%) in animals of experiment group in comparison with calvel of control group (4.01 and 7.31% respectively). Cell protection factors get active. Phagocytic activity of blood cells in the experiment animal was 16.5% higher in compare with control: 56% versus 49.8% respectively. Simultaneously, a statistically significant (p < 0.001) increase in blood neutrophil bactericidal activity was indicated of 57.09% in the experiment versus 46.43% in the control. Indicator of reserve capacity of bactericidal activity in the experimental group was 6.82% against 4.03% in the controls (p < 0.001). It was established that under the influence of probiotic ALS blood on the 10 and 20 day of use of a biological product was higher in calves of the experimental group and was equal to 56.06% and 57.09% against 42.12% and 46.43% in control, respectively. Conducted research and production experience, and the results obtained show that the use of the drug STF 1/56 TiM alimentary to calves of newborn period with milk has a statistically significant effect on hematologic and immunologic status of the animals.