West Beni Suef Concession is located at the western part of Beni Suef Basin which is a relatively under-explored basin and lies about 150 km south of Cairo. The major goal of this study is to evaluate the source rock by using different techniques as Rock-Eval pyrolysis, Vitrinite reflectance (%Ro), and well log data of some Cretaceous sequences including Abu Roash (E, F and G members), Kharita and Betty formations. The BasinMod 1D program is used in this study to construct the burial history and calculate the levels of thermal maturity of the Fayoum-1X well based on calibration of measured %Ro and Tmax against calculated %Ro model. The calculated Total Organic Carbon (TOC) content from well log data compared with the measured TOC from the Rock-Eval pyrolysis in Fayoum-1X well is shown to match against the shale source rock but gives high values against the limestone source rock. For that, a new model is derived from well log data to calculate accurately the TOC content against the limestone source rock in the study area. The organic matter existing in Abu Roash (F member) is fair to excellent and capable of generating a significant amount of hydrocarbons (oil prone) produced from (mixed type I/II) kerogen. The generation potential of kerogen in Abu Roash (E and G members) and Betty formations is ranging from poor to fair, and generating hydrocarbons of oil and gas prone (mixed type II/III) kerogen. Eventually, kerogen (type III) of Kharita Formation has poor to very good generation potential and mainly produces gas. Thermal maturation of the measured %Ro, calculated %Ro model, Tmax and Production index (PI) indicates that Abu Roash (F member) exciting in the onset of oil generation, whereas Abu Roash (E and G members), Kharita and Betty formations entered the peak of oil generation.