Dianthus caryophyllus is one of the most important species in Dianthus genus. In order to propagation virus-free plant material, in vitro culture of this species var cerise royallette were studied. Explants were cultured in Anderson medium containing 1 mgl-1 6-benzylaminopurine. 6-benzylaminopurine alone induced callus in nodes and internodes explants. To achieve organogenesis, calli subcultured onto Anderson medium with or without plant growth regulator. The highest shoot length was obtained in medium without any hormone. The most suitable adventitious shoot regeneration medium was Anderson medium supplement with 4 mgl-1 Kin and 0.1 mgl-1 NAA. Root regeneration was observ ed in medium with 0.5 and 1 mgl-1 naphtalenacetic acid. Rooted plantlet transferred into pot with perlite and irrigated by different nutrient solution. The effect of different nutrient solution (An, OM, MS, MC) on growth and development of plantlet were compared. The results showed Anderson medium was more effective than the others. Acclimatized plants were transferred to soil. All plants were survived in field condition and produced flower after 4 months.