
ABSTRACT. Batista L.C.S.O., Florencio C.N., Cid Y.P., Magalhães V.S., Chaves D.S.A. & Coumendouros K. [Bioprospecting extracts jaborandi against Ctenocephalides felis felis, Rhipicephalus sanguineus and Rhipicephalus microplus.] Bioprospecção de extratos de jaborandi contra Ctenocephalides felis felis, Rhipicephalus sanguineus e Rhipicephalus microplus. Revista Brasileira de Medicina Veterinária, 35(Supl.2):113- 118, 2013. Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veteriná- ria, Anexo 1, Universidade Federal Rural do Rio de Janeiro, Campus Seropédica, BR 465 Km 7, Seropédica, RJ 23897-970, Brasil. E-mail: katherinac@gmail.com The aim of this work was to evaluate the activity of Pilocarpus pennatifolius against Ctenocephalides felis felis, Rhipicephalus sanguineus and Rhipicephalus microplus. The in vitro assays proceeded according to the immersion test using engorged females (Drummond et al. 1973) opposite of R. microplus and R. sanguineus, and impregnation filter paper test for C. f. felis. The extract of P. pennatifolius was obtained by exhaustive maceration and their chemical composition was assessed by thin layer chromatography followed by colorimetric assays. Dilutions of the extract were prepared in the concentration range between 625 and 20,000 ppm. The results showed that the extract of jaborandi was activity in vitro in the range of concentration tested against ectoparasites reviews with efficacy values of 35% to C. f. felis, 31.82% to R. microplus and 59.06% to R. sanguineus.
O objetivo deste estudo foi avaliar a atividade de Pilocarpus pennatifolius frente à Ctenocephalides felis felis, Rhipicephalus sanguineus e Rhipicephalus microplus. A realização dos ensaios in vitro procedeu-se segundo o teste de imersão de teleóginas (Drummond et al. 1973) frente à R. microplus e R. sanguineus e teste de impregnação em papel filtro frente à C. f. felis. O extrato das folhas de P. pennatifolius foi obtido por maceração exaustiva e sua composição química foi avaliada por cromatografia em camada delgada seguido de ensaios colorimétricos. Foram preparadas diluições do extrato na faixa de concentração entre 625 e 20.000 ppm. Os resultados demonstraram que o extrato de jaborandi apresentou atividade in vitro na faixa de concentração testada frente aos ectoparasitos avaliados com valores de eficácia de 35% para C. f. felis, 31,82 % para R. microplus e 59,06% para R. sanguineus.
Pilocarpus, In vitro test, chromatography, Teste in vitro, cromatografia, ectoparasites, ectoparasitas
Pilocarpus, In vitro test, chromatography, Teste in vitro, cromatografia, ectoparasites, ectoparasitas
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