Recessive Dystrophic Epidermolysis Bullosa (RDEB) is a severe skin disease with no cure. Normally, two layer of the skin are held together by rope like structures called anchoring fibrils, which are made of Type VII Collagen (C7). However, in patients with RDEB the anchoring fibrils don’t work and the layers of the skin are fragile, resulting in blistering, chronic wounds, and cancer. RDEB is caused by mutations in the gene COL7A1, which makes C7. I aim to correct those COL7A1 mutations with new gene editing tools called base editor and prime editor, to fix the anchoring fibrils in patient cells. After editing COL7A1, I will use molecular techniques including Sanger sequencing, qPCR, western blot and immunofluorescent microscopy to confirm if the edit worked and if it fixed C7 function. It’s also important to know whether the gene editors have affected areas of the DNA which I don't want to edit, so I will do “off-target analysis” to test the safety of these tools. Additionally, I aim to make 3D models of "RDEB skin in a dish" to aid future research. By the end of my PhD I aim to have aided the development of potential cures for RDEB.