Chronic pain diseases affect 30% of the European population. Unfortunately, only 50% of patients receive appropriate alleviation, due to a lack of treatment specificity and efficacy, as a result of a current poor understanding of the underlying mechanisms. The project ‘PINCH’ is a transdisciplinary and innovative project, based on an integrated approach whose aim is to provide highly relevant information on the brain’s function during the experience of acute pain (in healthy animals) and aberrant hypersensitivity (in neuropathic animals). Using a highly innovative neuroimaging technique (fUS) in freely moving animals pioneered by the partners of this consortium, this project will determine selective biomarkers of acute/neuropathic pain, i.e. decipher the brain areas and brain networks differentially activated during the multiple components of acute or chronic pain. In addition, we will determine the specific alterations of brain networks concomitant with the emergence of either anxiety or depression. Finally, the last part of this project, proposes a general framework for the optimized and personalized treatment of chronic pain based on functional connectivity monitoring. In this proof-of-concept study, we propose to use individual analysis of functional connectivity assessed by ultrasound for a personalized pain relief strategy. Such approach is key for a future clinical transfer in chronic pain patients.