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35 años de la PCR, la técnica que revolucionó la biología molecular

Authors: Zardoya, Rafael;

35 años de la PCR, la técnica que revolucionó la biología molecular

Abstract

[ES] La reacción en cadena de la polimerasa o PCR permite amplificar de forma exponencial un fragmento de ADN específico. Esta sencilla pero potente técnica aprovecha la capacidad natural de las ADN polimerasas de copiar las dos hebras de un ADN molde mediante la incorporación de nucleótidos a partir de un par de cebadores. Tiene numerosas aplicaciones en biología.

[EN] The polymerase chain reaction or PCR allows amplifying exponentially a specific DNA fragment. This simple but powerful technique is based on the natural capacity of DNA polymerases to copy the two strands of a template DNA through the incorporation of nucleotides starting from a pair of primers. It has numerous applications in biology.

[ES] The polymerase chain reaction or PCR allows amplifying exponentially a specific DNA fragment. This simple but powerful technique is based on the natural capacity of DNA polymerases to copy the two strands of a template DNA through the incorporation of nucleotides starting from a pair of primers. It has numerous applications in biology.

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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