N50 for assembly results from different assembly strategies assembled by IDBA-UD and SPAdes. N50 values for the de novo assembly results for E. coli DH1 and S. Parasanguinis FW213 by IDBA-UD and SPAdes. Group A [PE] and Group A [SE] represent all reads assembled as paired-end reads and single end reads, respectively. Group A [PEâ +â SE] represents all the non-overlapped paired-end reads assembled together with merged reads. Group M [PE] and Group M [SE] represent Group M reads assembled as paired-end reads and single end reads, respectively. Group M slightly outcompete Group A when depth of read coverage is low. However, Group A always has the highest N50 values when depth of read coverage is high. (TIFF 867Â kb)