Fig. 3. FT-IR analysis of wood in transgenic hybrid aspen lines expressing 35S::SPCel9B3:PcCGE. (A) OPLS-DA models of diffuse reflectance FT-IR spectra from wood of transgenic trees (grey symbols, n = 7–10 per line, five lines) and wild-type (WT) trees (black symbols, n = 18). The model shows the separation between the transgenic and WT plants with one predictive and two orthogonal components. (B) Correlation-scaled loadings plot for Predictive Component 1, showing factors separating transgenic and WT trees. The marked positive bands are more intense in WT and related to cellulose and other carbohydrates. The marked negative bands are more intense in transgenic plants and are mostly related to lignin. The major separating factors that are marked with arrows in the Loadings plots are summarized in Supplementary Table S1.