Splicing factors (SFs) act in dynamic macromolecular complexes to modulate RNA processing. To understand the complex role of SFs in cancer progression, we performed a comprehensive analysis of the co-regulated gene expression of SFs in cancer cells. Our results show that SFs are involved in the regulation of various cellular processes, including cell proliferation, apoptosis, and migration. The study highlights the importance of SFs in cancer progression and suggests that targeting SFs may be a promising therapeutic strategy. Furthermore, the study provides insights into the complex mechanisms underlying SFs regulation in cancer cells, which may lead to the development of novel cancer therapies.