Haemaphysalis bispinosa Neumann has been considered to exist in China, especially in the southern part of the country. However, H. bispinosa referred to in many Chinese research papers may in fact be misidentified. This study aims to investigate the presence and distribution of H. bispinosa in China, and to clarify the taxonomy and molecular characteristics of this species. The results of this study will provide valuable information for the prevention and control of tick-borne diseases in China.