The size of skeletal muscle cells is precisely regulated by intracellular signaling networks that determine the balance between overall rates of protein synthesis and degradation. Myofiber growth and maintenance are tightly controlled by a complex interplay of transcription factors, growth factors, and signaling pathways. The JunB transcription factor plays a crucial role in regulating muscle cell size by modulating the expression of genes involved in protein synthesis and degradation. This study aimed to investigate the role of JunB in maintaining skeletal muscle cell size and its potential implications for muscle wasting disorders.