Iron is an essential nutrient for almost all living organisms. Almost every cell requires iron to produce energy. However, its supply is often limited, and too much iron is toxic and can cause death. Therefore, many cells have mechanisms to sense, use and storage to adapt to it’s changing availability. Cells that are infected with pathogens will often restrict iron availability to limit the survival of the pathogen. In response to this, many pathogens have developed sophisticated ways to subvert iron from the host to survive. During my PhD, I will be looking at how the parasite Toxoplasma gondii acquires iron from the host cell, and to look at the interplay and dynamics of iron-related proteins in the host. I will also investigate if, and how, the parasite modulates iron uptake, regulation and utilisation in host cell following infection. To study these processes, I aim to use a number of genetic, biochemical, and advanced imaging techniques.