[ES]: Se obtuvieron fragmentos de schwannomas vestibulares (EV) humanos tras una intervención quirúrgica en el Hospital Universitario de la Paz, Madrid, España. Se almacenaron en condiciones específicas para obtener ARN. Junto con estas muestras se analizó un ARN comercial de células de Schwann (SC). El análisis bioinformático comenzó con el mapeo de las lecturas de secuenciación a un transcriptoma de referencia, luego se cuantificaron los niveles de expresión de genes individuales y, finalmente, se identificaron genes específicos que se expresaban diferencialmente entre las muestras de VS y SC.

[EN]: Fragments of human vestibular schwannomas (VS) were obtained after surgery at the Hospital Universitario de la Paz, Madrid, Spain. They were stored under specific conditions to obtain RNA. A commercial Schwann cell (SC) RNA was analysed together with these samples. The bioinformatic analysis began by mapping the sequencing reads to a reference transcriptome, then quantifying the expression levels of individual genes, and finally identifying specific genes that were differentially expressed between VS samples and SC.

Data collection was funded by Spanish MCIN/ AEI/ 10.13039/ 501100011033 THEARPY-PID2020-115274RB-I00, MINA-CM Madrid Innovative Neurotech Alliance P2022/BMD-7236, CA20113 COST Action EU- ProteoCure, CA23119 COST Action EU-SENESCENCE2030: Targeting Cell Senescence to Prevent Age-Related Diseases and the Spanish Senescence Network grants to Isabel Varela Nieto.

Vestibular Schwannoma fragments kept in RNAlater at -80°C were treated with Qiazol lysis reagent for RNA extraction using RNeasy mini kit (Qiagen, 50974106) following the instructions recommended by the manufacturer. Human Schwann Cell RNA (#1705, ScienCell, Carlsbad, CA, USA) was used as control condition.

Genes_expresados_05_Tumor_SC.xlsl

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