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FICHERO X204SC24075390-Z01-F003_3.tar 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FICHERO X204SC24075390-Z01-F003_4.tar ##04.Ref/genome.gtf.gz04.Ref/genome_gene.fa.gz04.Ref/genome.fa.gz

Los datos depositados contienen los resultados de la secuenciación masiva del ARN total aislado a partir de 63 schwannomas vestibulares, 4 schwannomas de nervios distintos al nervio vestibular (VS11, VS33, VS34 y VS74) y 7 fragmentos de diferentes nervios sanos (VS56, VS58, VS65, VS66, VS67, VS68, VS99). Los fragmentos de VS conservados en RNAlater® a -80°C se trataron con el reactivo de lisis Qiazol para la extracción de ARN utilizando el mini kit RNeasy (Qiagen, 50974106) siguiendo las instrucciones recomendadas por el fabricante en el servicio de Génomica del Instituto de Investigaciones Biomédicas Sols-Morreale de Madrid. Se utilizó ARN con un RIN (índice de integridad del ARN) superior a 7 para la secuenciación. La secuenciación se llevó a cabo utilizando la secuenciación PE150 (secuenciación “paired-end” de 150 pb en cada hebra) en la plataforma Illumina NovaSeq X Plus (Illumina, San Diego, CA, EE.UU.) de la empresa Novogene. Los datos de secuenciación depositados se presentan en formato fastq.

[Description of methods used for collection/generation of data] Sequencing was performed using PE150 sequencing (150 bp paired-end sequencing on each strand) on the Illumina NovaSeq X Plus platform (Illumina, San Diego, CA, USA) from Novogene. Files are row data.

This research was funded by Spanish MCIN PID2020-115274RB-I00 (THEARPY), PID2023-147347OB-I00 (PROHEAR), and CSIC #2024AEP115 grants to Isabel Varela-Nieto and Silvia Murillo-Cuesta

Peer reviewed