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handle: 10261/365629
Additional file 1: Figure S1. A minimal increase in Cdc20Slp1 levels causes sensitivity to MBC and defective mitotic arrest. A. Slp1 protein level was determined from asynchronously growing cells of the indicated genotypes. Cdc2 was used as a loading control. B. Spot growth assay of the indicated strains and condition. C-E. Representative examples of mitotic arrest assay of the indicated strains. Cell cultures were grown at permissive temperature (32ºC) and then shifted to restrictive temperature (18ºC). Samples were taken every hour and the septation index calculated form more than 200 cells in each time point and condition. Representative examples of two independent experiments are shown.
Ministerio de Economía y Competitividad Ministerio de Ciencia e Innovación Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía Fundación Séneca de la Región de Murcia National Institutes of Health
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MCC, Osmotic stress, Pmk1, CIP, APC/C, Mitosis, SAPK, Sty1, CDC20/Slp1, MAPK, Spindle assembly checkpoint, Cytokinesis
MCC, Osmotic stress, Pmk1, CIP, APC/C, Mitosis, SAPK, Sty1, CDC20/Slp1, MAPK, Spindle assembly checkpoint, Cytokinesis
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