Additional file 2: Supplementary Figure S2. Regulation of METTL1 expression in PCa. A) Correlation analysis between METTL1 and KLK3 expression, and METTL1 and AR expression in the human primary PCa expression datasets. The plotted values correspond to the log2-normalised expression values. Black line represents linear regression, grey area indicates the limits of the confidence intervals. Pearson's correlation coefficient (R) and p-values are indicated. Grasso n = 88; Taylor n = 183; TCGA n = 497. B) No effect on mRNA expression levels of METTL1 upon dihydrotestosterone (DHT) treatment in LNCaP cells. Mean ± SD, n = 3. C) Graphical representation of PI3K inhibition by different compounds. Small molecule inhibitors used: pan-PI3K inhibitor BKM-120 (BKM), AKT inhibitor MK2206 (MK), mTORC1 inhibitor rapamycin (RAPA), and mTORC1/2 inhibitor Torin (TOR). D, E) METTL1 expression is affected by the inhibition of downstream PI3K pathway members. Western blotting (D) and RT-qPCR (E) analyses of METTL1 expression upon PI3K pathway inhibition in PC3 cells. The cells were treated for 48 h (D) or 8 h (E). Mean ± SD, n = 2 (D) and n = 6 (E). F) Representative images of immune stained sections for Mettl1 and markers for luminal (AR), and basal (K14) cells in Pten-KO prostates (dorsal and ventral lobes) in invasive carcinoma (6 months old mice) and in aged-match wild-type (WT) prostates. Scale bars represent 50 μm. Statistical tests: One-tailed Student’s t-test (B, D, E). *p < 0.05, **p < 0.01, ***p < 0.001.

Consejo Superior de Investigaciones Cientificas (CSIC)

Peer reviewed