Functional characterization and subcellular localization of teleost wild-type Aqp1ab1 and -1ab2 and their splice variants [Dataset]

Ferré, Alba; Chauvigné, François; Zapater, Cinta; Finn, Roderick N.; Cerdà, Joan

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Dataset . 2024 . Peer-reviewed

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Functional characterization and subcellular localization of teleost wild-type Aqp1ab1 and -1ab2 and their splice variants [Dataset]

Research datakeyboard_double_arrow_right Dataset 01 Jan 2024 English Publisher:Plos One

Authors: Ferré, Alba; Chauvigné, François; Zapater, Cinta; Finn, Roderick N.; Cerdà, Joan;

handle: 10261/361324

Functional characterization and subcellular localization of teleost wild-type Aqp1ab1 and -1ab2 and their splice variants [Dataset]

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Abstract

(A-C) Pf of X. laevis oocytes injected with water (W, controls) or expressing seabream SaAqp1ab1-WT, SaAqp1ab1_v1 or SaAqp1ab1_v2 (A), Atlantic halibut HhAqp1ab1-WT or HhAqp1ab1_v1 (B), or sole SsAqp1ab2-WT, SsAqp1ab2_v1 or SsAqp1ab2_v2 (C). Oocytes injected with SsAqp1ab2-WT or splice forms were co-injected with halibut YwhazLb and exposed to FSK for 1 h prior to the swelling assay. The data are the mean ± SEM (n indicated above each bar) and were statistically analyzed by an unpaired Student’s t-test (***, P < 0.001; with respect to controls). (D-F) Double immunostaining of oocytes expressing untagged SaAqp1ab1-WT or HA-tagged SaAqp1ab1_v1 or HA-SaAqp1ab1_v2 (D), Flag-tagged HhAqp1ab1-WT or HA-tagged HhAqp1_v1 (E), or Flag-tagged SsAqp1ab2-WT or HA-tagged SsAqp1ab2_v1 or HA-SsAqp1ab2_v2 (F), and the ER marker protein disulfide isomerase (PDI). The plasma membrane is indicated by an arrowhead, whereas the co-localization of aquaporin channels and PDI in the cytoplasm is indicated by arrows. Scale bars, 10 μm (insets 5 μm).

With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S).

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Keywords

Truncated proteins, aqp1ab2 isoforms, Marine teleosts, Aqp1ab2 may represent, Maximal egg hydration, Osmoregulatory systems develop, Inhibition likely occurs, Membrane trafficking, Truncated aqp1ab2, Canonical channels, Thus leaving, Vital water, 1 cluster, Negative inhibition, Ultimate degradation, Oocyte hydration, Also partially escape, Canonical channel trafficking, Canonical channel, Duplicated ywhaz, Truncated protein, Meiosis resumption, Differentially control, Binding proteins, Cause dominant, Specific aquaporin, New regulatory mechanism, Inhibitory isoforms, Findings suggest, Type variants, Isoforms cause dominant, Intact protein, Degradation pathway, Exon readthrough, Marine teleosts causes, Two water channels, Mediated oocyte hydration, Physiologically modulated, Oocyte plasma membrane, Negative enhancement, aqp1ab2 channel, Recent studies, Xink "> aquaporin, Endoplasmic reticulum, Plasma membrane, Subsequently highest, Early embryos

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