A–D) Schematic drawings of early S9 (A), middle S9 (B), late S9 (C), and S10 egg chamber illustrating the BCs (yellow), NCs (gray), FCs (purple), BM (green), and the point of ablation in the NCs (blue bar). (A’–D’) Images of life control egg chambers of the indicated developmental stages expressing Resille-GFP before and after NC bonds are ablated. Blue bars indicate points of ablation. (E) Schematic representation of an egg chamber and the point of ablation in NCs. (F, G) Quantification of the initial velocity of vertex displacement of the indicated ablated bonds. The statistical significance of differences was assessed with a t test, * P value < 0.05 and ** P value < 0.01. Horizontal and vertical lines indicate mean and SD, respectively. Scale bar in A’–D’, 10 μm. The raw data underlying panels F and G are available in S1 Data.

Peer reviewed