(A) Ribbon representation of the two 3Dpol molecules present in the asymmetric unit of the P212121 crystals (yellow and green ribbons for the 3Dpol I and 3Dpol II molecules, respectively). The 3B1 molecule bound to the bottom of the palm of the two 3Dpol molecules is shown as sticks in atom-type colour (carbons white). The ordered triphosphate moieties of the UTP molecules bound at the nucleotide-binding site of the polymerases are also shown in atom-type sticks (phosphates in orange). (B) Omit map around the 3B1 molecule, displayed at a contour of 1,0 σ (light blue mesh). 3B1 is shown in sticks as in A with the amino acids explicitly labelled. (C) Details of the intermolecular contacts stablished between 3Dpol I and the 3B1 N-terminus (Interface I). (D) Intermolecular interactions between 3Dpol II and the 3B1 C-terminus (Interface II). The interacting residues are shown in sticks representation, coloured as in A. Hydrogen bonds and salt bridges are shown as dashed lines in black.

Peer reviewed