Downloads provided by UsageCountsLoading controls for the in vitro assembly reactions
Méndez-López, Eduardo; Aranda, Miguel A.;
Loading controls for the in vitro assembly reactions
Western blot of a protein extract from N. benthamiana leaves expressing PepCPWT. Protein extract aliquots were oxidized with CuCl2 (lanes 1 and 2), reduced with dithiothreitol (lanes 3 and 4), or not pre-treated with any of them (lanes 5–6). Protein extracts loaded in lanes 2, 4 and 6 were incubated with methyl-polyethylene glycol-maleimide (MM(PEG)24) as described by Pant et al. (2021). Reduced cysteines are alkylated with MM(PEG)24 and one conjugation increases the protein mass by 1.24 kDa. A double band was observed in lanes 2 and 4, indicating partial CP oxidation, reduction or alkylation in the controls. The membrane was stripped and re-probed with an anti-Actin antibody as the loading control
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Plant infections, Results strongly suggest, Wt, Binding pocket, Like particles, Div >< p, Rna binding pocket, Cell movement, 200 nm could, Animal virus infections, Vivo </, Cysteine oxidation, Electron microscopy, Viral cell, Viral rna, Dispersed vlps, Single cysteine residue, Plant virus protein, Redox modifications, Coat protein, Planta </, C127s, Via </, Vlps longer, Vitro </, Tomato crops, Glutathionylation blocks, Identified critical residues, Redox status, Vlps )., Regulatory role, Highly conserved, Cp rna
Plant infections, Results strongly suggest, Wt, Binding pocket, Like particles, Div >< p, Rna binding pocket, Cell movement, 200 nm could, Animal virus infections, Vivo </, Cysteine oxidation, Electron microscopy, Viral cell, Viral rna, Dispersed vlps, Single cysteine residue, Plant virus protein, Redox modifications, Coat protein, Planta </, C127s, Via </, Vlps longer, Vitro </, Tomato crops, Glutathionylation blocks, Identified critical residues, Redox status, Vlps )., Regulatory role, Highly conserved, Cp rna
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This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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