Verticillium wilt of olive (VWO), caused by Verticillium dahliae, is a major concern in many olive-growing countries. An efficient VWO control measure is the use of tolerant/resistant cultivars. Low information is available about olive secondary metabolites and its relationship with VWO tolerance. In this study, a comprehensive metabolic profiling of the roots of six olive cultivars differing in their level of tolerance/susceptibility to VWO was addressed. Potential changes in the metabolite profiles due to the presence of the pathogen were also assessed. A strong relationship between the quantitative basal composition of the root secondary metabolic profile and VWO tolerance/susceptibility of olive varieties was found. Tolerant cultivars showed higher content of secoiridoids, while the susceptible ones presented greater amounts of verbascoside and methoxypinoresinol glucoside. The presence of V. dahliae only caused few significant variations mostly restricted to the earliest times after inoculation. Thus, a rapid activation of biochemical-based root defense mechanisms was observed. Key policy highlights Quantitative differences of secondary metabolites in roots contribute to explain the tolerance/susceptibility of olive cultivars to Verticillium dahliae. Higher basal content of secoiridoids correlate with tolerance, while greater concentration of verbascoside and methoxypinoresinol glucoside seem to be linked to susceptibility. Few alterations are observed in the olive root metabolic profiles in the presence of the pathogen. Changes in the root metabolic profile occur at early times after pathogen inoculation which suggests a rapid activation of a biochemical-based defense response against V. dahliae. Quantitative differences of secondary metabolites in roots contribute to explain the tolerance/susceptibility of olive cultivars to Verticillium dahliae. Higher basal content of secoiridoids correlate with tolerance, while greater concentration of verbascoside and methoxypinoresinol glucoside seem to be linked to susceptibility. Few alterations are observed in the olive root metabolic profiles in the presence of the pathogen. Changes in the root metabolic profile occur at early times after pathogen inoculation which suggests a rapid activation of a biochemical-based defense response against V. dahliae.

This work was supported by the grants PID2019-106283RB-I00, BES-2017-081269 and FPU19/00700 of the Spanish Ministerio de Ciencia, Innovación y Universidades (MICIU)/Agencia Estatal de Investigación (AEI), and the grant RYC2021-032996-I funded by MCIN/AEI/10.13039/501100011033 and by “European Union NextGenerationEU/PRTR”. This research was partially funded by FEDER/Junta de Andalucía-Consejería de Conocimiento, Investigación y Universidad, Junta de Andalucía Transformación Económica, Industria, Conocimiento y Universidades, Proyecto P20_00263; and FEDER/Junta de Andalucía-Consejería de Economía y Conocimiento, Proyecto B-AGR-416-UGR18.

Peer reviewed