Powered by OpenAIRE graph
Found an issue? Give us feedback
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Recolector de Cienci...arrow_drop_down
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
Recolector de Ciencia Abierta, RECOLECTA
Dataset . 2023 . Peer-reviewed
Data sources: Recolector de Ciencia Abierta, RECOLECTA
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
DIGITAL.CSIC
Dataset . 2023 . Peer-reviewed
Data sources: DIGITAL.CSIC
 View all 2 versions
Claim

Electronic Supplementary Material Determination and localization of specific proteins in individual ARPE-19 cells by single cell and laser ablation ICP-MS using iridium nanoclusters as label

Research datakeyboard_double_arrow_right Dataset 01 Jan 2023 Spain English Publisher:Elsevier BV
Authors: Menero-Valdés, Paula; Lores-Padín, Ana; Fernández-Vega, Beatriz; Quarles Jr., C. Derrick; García, Montserrat; González-Iglesias, Héctor; Pereiro, Rosario;

handle: 10261/339069

Electronic Supplementary Material Determination and localization of specific proteins in individual ARPE-19 cells by single cell and laser ablation ICP-MS using iridium nanoclusters as label

Abstract

This Electronic Supporting Material contains details about the Experimental section as well as some additional comments about Results and Discussion section. The Experimental section describes the reagents employed for the synthesis and characterization of the IrNCs and IrNCs-immunoprobes, the culture and incubation of ARPE-19 cells, the immunocytochemistry (ICC) assays for the detection of target proteins by MS and fluorescence, the cellular preparation for sc-ICP-MS and LA-ICP-MS, as well as the ELISA analyses. Furthermore, protocols for the synthesis and characterization of IrNCs and IrNCs-immunoprobes are also included. In the Results and Discussion section, results regarding the optimization of the glucose-induced oxidative stress treatment, the determination of proteins in individual ARPE-19 cells by sc-ICP-MS, as well as the bioimaging of APOE and claudin-1 in individual ARPE-19 cells by LA-ICP-MS are also collected.

Peer reviewed

Country
Spain
Related Organizations
  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 0
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
    OpenAIRE UsageCounts
    Usage byUsageCounts
    visibility views 68
    download downloads 28
  • 68
    views
    28
    downloads
    Powered byOpenAIRE UsageCounts
Powered by OpenAIRE graph
Found an issue? Give us feedback
visibility
download
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
downloads
OpenAIRE UsageCountsDownloads provided by UsageCounts
0
Average
Average
Average
68
28