S1. Sample preparation protocol for evaluation of OneFlow kits (BD). Figure S1. Impact of different BSA concentrations and different pH of the washing buffer on the relative percentage distribution of different cell populations identifiable in normal PB samples with LST. Figure S2. Impact of different BSA concentrations and different pH of the washing buffer on the expression levels of individual LST markers as assessed on different cell populations in normal PB samples. Table S1. Detailed information on the antibody reagents used in the current study, including the antibody titer used per test. Table S2. Absolute and relative percentage changes of particular cell populations, debris and doublets detectable in BM samples stained with ALOT tube at day 0 and after 24-h storage. Table S3. Ratios of percentage of debris, doublets, and pathological/clonal B-cells in particular BM and PB samples stained with LST tube at different time points. Table S4. Ratios of percentage of debris, doublets, and pathological/clonal B-cells in particular BM and PB samples stained with the first tube of B-CLPD panel at different time points. Table S5. Ratios of percentage of debris, doublets, and pathological/clonal plasma cells in particular BM and PB samples stained with PCD panel at different time points. Table S6. MFI values of LST markers evaluated on relevant cell populations obtained at different pH and BSA concentrations. Table S7. Expression of markers on relevant PB cell populations stained with LST tube at different pH on averaging the results for different BSA concentrations. Table S8. Selected additional markers that were not directly studied in the current study that may be influenced by the preparation-associated variables.

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