Fig. S1. Genetic context of the tannase gene (FN_0616) within the F. nucleatum subsp. nucleatum 25586 genome. Fig. S2. TanBFnn is monomeric in solution. Fig. S3. TanBFnn substrate specificity. Fig. S4. Biochemical properties of TanBFnn. Fig. S5. Amino acid sequence and active site comparisons between TanBFnn and TanALp. Fig. S6. Representative snapshots of three different 1 microsecond MD simulations of ethyl gallate (EG, in red) bound to TanBFnn. Fig. S7. Representation of MD simulations of methyl digallate (MDG, in red) bound to TanBFnn. Fig. S8. SPD interacts with TanBFnn. Fig. S9. Structure of spermidine and its N1 and N8-acetylated derivatives in their charged form at neutral pH. Fig. S10. Multiple sequence alignment of TanBFnn with homologs found within the Fusobacteriales, particularly, from the genus Leptotrichia: Leptotrichia sp. OH3620_COT-345 (WP_125237392.1); L. wadei (WP_146961278.1); L. massiliensis (WP_071123737.1); L. buccalis (WP_157859547.1). Fig. S11. Multiple sequence alignment of TanBFnn with homologs from bacterial genera that are prevalent in the oral cavity: Aggregatibacter (WP_006718097.1); Selenomonas (WP_051586482.1); Veillonella (WP_127058572.1); Mitsuokella (WP_118475533.1). Fig. S12. Multiple sequence alignment of TanBFnn with homologs from bacterial genera present in the human gut: Leptotrichia (WP_157859547.1); Veillonella (WP_127058572.1); Megasphaera (WP_044503365.1); Roseburia (WP_173884414.1); Bacteroides (WP_164733466.1); Parabacteroides (WP_172733427.1); Lachnoclostridium (WP_087219107.1); Fusicatenibacter (WP_173816499.1); Dorea (WP_161159564.1); Blautia (WP_095175021.1); Lactobacillaceae (WP_054397147.1); Sutterella (GenBank: RGU74189.1). Table S1 related to Figure 1. Species harboring orthologous tannase proteins.

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