Between 2010 and 2013 we collected red colobus samples from eight sites in or near Kibale National Park. We sampled three sites within Kibale (Kanyawara, Sebitoli, and Mainaro) and five fragments (Isunga, Kamakune, Byara, Lake Nkuruba, and Lake Kasenda). Of the collected samples, some were blood samples from Kanyawara and the rest were fecal samples across the other localities. Fecal samples were collected along transects except for Kanyawara where samples were collected during behavioral surveys from habituated, known individuals. All samples were genotyped at 15 microsatellite loci selected from the human genome and that have been used in previous studies of colobus monkeys Alleles were scored using GeneMapper® Software 5 (Applied Biosystems, Foster City, CA, USA). Heterozyogous genotypes were confirmed in at least two separate reactions and homozygous genotypes were confirmed in at least three separate reactions. Blood samples were genotyped twice, and fecal samples were genotyped from 3 to 6 times (see above). We assigned a consensus genotype per sample across runs and then calculated the error rate per locus, including both false allele and allelic drop out, using the program GIMLET (Valière, 2002).

Date of data collection: Samples collected in 2013, Genotyped between 2013 and 2015.-- Number of variables: 15 micro satellite markers.

NIH: TW009237, NSF BCS-1540459.

RCMicrosatellitegenotypesKibale

Peer reviewed