The study focuses on the comparison of susceptible and benzimidazole-resistant Haemonchus contortus adult females and males. The dataset includes NMR spectra from four experimental groups (n=10): ISE-M (sensitive males), ISE-F (sensitive females), IRE-M (resistant males), IRE-F (resistant females). The proton spectra of polar extracts from individual samples were acquired on a 600 MHz Bruker Avance III spectrometer using CPMG pulse sequence (cpmgpr1d).