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Overview Chlamydomonas reinhardtii theoretical signal peptides identified by SignalP 4.0 in a protein data set described below: Protein data set came from "The Genome Portal of the Department of Energy Joint Genome Institute" (http://genome.jgi.doe.gov/) Protein sequences were evaluated in SignalP 4.0 Server (http://www.cbs.dtu.dk/services/SignalP/) File used Chlre4_best_proteins.fasta.gz -> Protein dataset version used for analysis Workflow ______Chlre4_best_proteins.fasta.gz_______ | | Chlre4_best_proteins_fasta_protein_woSP.fasta Chlre4_best_proteins_signalPeptide.fasta | ___Chlre4_best_proteins_signalPeptide_unique.fasta___ | | Chlre4_best_proteins_signalPeptide_unique.aln Signal Peptide Anotation from aligned.xlsx Info Chlre4_best_proteins_fasta_protein_woSP.fasta -> Mature protein sequences from proteins identified without signal peptide Chlre4_best_proteins_signalPeptide.fasta -> Identified signal peptide Chlre4_best_proteins_signalPeptide_unique.fasta -> Unique identified signal peptide Chlre4_best_proteins_signalPeptide_unique.aln -> Align signal peptides (UGENE) Signal Peptide Annotation from aligned.xlsx -> Signal peptide list, highlighted in orange theoretical tested. Citations For use of signal peptide dataset, please cite: Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433 and SignalP 4.0: discriminating signal peptides from transmembrane regions Thomas Nordahl Petersen, Søren Brunak, Gunnar von Heijne & Henrik Nielsen Nature Methods, 8:785-786, 2011 doi: 10.1038/nmeth.1701 PMID: 21959131 Supplementary materials: nmeth.1701-S1.pd and The genome portal of the Department of Energy Joint Genome Institute: 2014 updates H. Nordberg, M. Cantor, S. Dusheyko, S. Hua, A. Poliakov, I. Shabalov, T. Smirnova, I. V. Grigoriev, I. Dubchak, , Nucleic Acids Res. 42, 26–31. 2014 doi:10.1093/nar/gkt1069.