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Reference datasets (Feb 2021 update with bug fixes) for Mitohelper (https://github.com/aomlomics/mitohelper) Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to the Mitohelper's DevWiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Feb2021.tsv (668,366 records; Jan 2021 MitoFish release) mitofish.12S.Feb2021.tsv (37,314 records) mitofish.COI.Feb2021.tsv (224,712 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza
Fixed irregular column breaks in Jan 2021 reference dataset. No records added or removed compared to Jan 2021.
fish, metabarcoding, eDNA
fish, metabarcoding, eDNA
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