This record contains (i) original replicate-level datasets (Excel) and (ii) the official statistical report issued by INTA–EEA Balcarce (PDF) supporting the bioassays reported in Sauka et al. (2026), Journal of Invertebrate Pathology. The files include in vitro assays against the plant-parasitic nematodes Meloidogyne javanica and Pratylenchus zeae. The datasets report the number of individuals evaluated per replicate and the number of dead or immobilized individuals per treatment and time point. Files are provided in Spanish as originally recorded. An English-formatted summary table is provided as Supplementary Material in the associated manuscript.