About CBASSED50 R package to process CBASS-derived PAM data. Minimal requirements are PAM data (or data from any other continuous variable that changes with temperature, e.g. relative bleaching scores) from 4 samples (e.g., nubbins) subjected to 4 temperature profiles of at least 2 colonies from 1 coral species from 1 site. Please refer to the CBASS method paper for in-depth information regarding CBASS acute thermal stress assays, ED50 thermal thresholds, etc. Evensen, N. R., Parker, K. E., Oliver, T. A., Palumbi, S. R., Logan, C. A., Ryan, J. S., Klepac, C. N., Perna, G., Warner, M. E., Voolstra, C. R., & Barshis, D. J. (2023). The Coral Bleaching Automated Stress System (CBASS): A low‐cost, portable system for standardized empirical assessments of coral thermal limits. Limnology and Oceanography, Methods / ASLO, 21(7), 421–434. https://doi.org/10.1002/lom3.10555 Get Started You can install the latest version of CBASSED50 from GitHub: if(!require(devtools)){ install.packages("devtools") } devtools::install_github("reefgenomics/CBASSED50") An example demo file is available here. Please, have a look at README.md for further details. Get Help You can always report the GitHub issue or email the current maintainer: yulia_iakovleva@proton.me. Contribute If you want to contribute to a project and make it better, your help is very welcome. You can always report an issue or fork this repository, implement/fix your feature, and create a pull request.

If you use this software, please cite it as below: Yulia Iakovleva & Christian R Voolstra. (2023). CBASSED50: R package to process CBASS-derived PAM data. Zenodo. https://doi.org/10.5281/zenodo.8370644.